New synthetic sugar-nucleotide donor substrates for glycosyltransferases

ORGN 275

Maria R. Manzoni, mmanzoni@ncifcrf.gov1, Elizabeth Boeggeman, eeb@helix.nih.gov2, Boopathy Ramakrishnan2, Marta Pasek1, and Pradman Krishen Qasba1. (1) Structural Glycobiology Section, Nanobiology Program, Center for Cancer Research, National Cancer Institute-NIH, P.O Box B, Bldg. 469, Rm 221, NCI-Frederick, Frederick, MD 21702-1201, (2) Structural Glycobiology Section, Nanobiology Program, Center for Cancer Research,SAIC, Inc, National Cancer Institute-NIH, P.O Box B, Bldg. 469, Rm 221, NCI-Frederick, Frederick, MD 21702-1203
Considering the important role of glycoconjugates in biological recognition, acute and chronic diseases (such as inflammation), and numerous cancer types; methods capable of introducing a sugar residue with a reactive chemical handle at a unique site in the oligosaccharide chain of relevant glycoprotein and/or glycolipid are highly desirable. Structure-based design of novel glycosyltransferases is making it possible to transfer unnatural sugars to specific monosaccharide residue on a glycan chain. These carbohydrates with chemical handles have been shown to be new sugar-donor substrates for natural and engineered mutant glycosyltransferases. While exploiting this selective, chemoenzymatic approach, these functionalized, unnatural glycoconjugates have a variety of new diagnostic and therapeutic applications allowing for the incorporation of probes, biomarkers, and/or drug-targeting systems.
 

Biologically Active Molecules
8:00 AM-12:00 PM, Tuesday, April 8, 2008 Morial Convention Center -- Rm. R05, Oral

Division of Organic Chemistry

The 235th ACS National Meeting, New Orleans, LA, April 6-10, 2008