ORGN 442 |
| Cysteine (Cys) detection is of great interest currently for playing crucial roles in physiological matrices. There have been some fluorescent sensors designed to afford thiazolidine by the reaction of aldehyde group with Cys, in which only Tanaka et al. have described a fluorescent turn-on sensor. Here we report a novel sensor RG based on Rhodamine 6G employing the same reaction of aldehyde group with Cys. We found that the formation of thiazolidine induced the “ring-open” of sensor RG and 10-fold fluorescence enhancement could be acquired at neutral pH upon addition 20 eq. Cys. Both the excitation and emission wavelength maximum are in visible light range. Furthermore, RG displays superior selectivity for Cys while other common amino acids are all inactive in our assay.
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Heterocycles and Aromatics, Molecular Recognition and Self Assembly
8:00 PM-10:00 PM, Tuesday, April 8, 2008 Morial Convention Center -- Hall A, Poster
Sci-Mix
Division of Organic Chemistry |
