ORGN 490 |
| The redox response of a magnetic resonance imaging (MRI) probe (spirooxazine-Gd-DO3A) was tested in water using a series of biological redox agents. NADH was found to be an effective reducing agent for the “open” isomer of the probe. The influence of NADH on the MR and optical imaging of the probe was tested in both solution and cells in culture. When in dark, the probe (open form) has a r1 relaxivity of 4.72 mM-1s-1 and gives out strong fluorescence. Upon mixing with NADH, the compound undergoes an isomerization to closed form. The r1 relaxivity increases by 41% and the brightness in MRI increases immediately and significantly. However, the fluorescence of the probe was almost fully quenched by NADH, which leads to an obvious intensity decrease in confocal images. The probe is promising for direct, non-invasive observation of biochemical process in vivo. |
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Materials, Devices and Switches
1:00 PM-4:40 PM, Wednesday, April 9, 2008 Morial Convention Center -- La Nouvelle, Blrm. C, Oral
Division of Organic Chemistry |