ORGN 771 |
| Continuous G-rich oligonucleotides can form G-quadruplex structures containing two or more guanine tetrads. The discovery of the telomeric sequences at the end of chromosomes has stimulated considerable interest in the studies of G-quadruplex. Oligonucleotides with only a single G-rich segment usually form parallel quadruplex through tetramerization of four strands, in which all of them adopt the same polarity. The synthesis and characterization of a series of G-rich DNA conjugates possessing one poly(G) segment and a pyrene alcohol linker at 5' position have been studied. The formation of pyrene probed quadruplex shows strand dependence. Both pyrene monomer and excimer fluorescence are observed for the G-quadruplex structures. The excimer/monomer intensity ratio for the G quadruplex structures is dependent upon both the temperature and potassium or lithium salt concentration. |
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Physical Organic Chemistry: Calculations, Mechanisms, Photochemistry, and High Energy Species
8:00 AM-12:00 PM, Thursday, March 29, 2007 McCormick Place Lakeside -- Room E451A/B, Level 4, Oral
Division of Organic Chemistry |