A universal chemoenzymatic method for nanostructure assembly

ORGN 388

Benjamin P Duckworth, duckworth@chem.umn.edu1, Juhua Xu1, T. Andrew Taton1, and Mark D. Distefano, diste001@umn.edu2. (1) Department of Chemistry, University of Minnesota, 207 Pleasant St SE, Minneapolis, MN 55455, (2) Departments of Chemistry and Medicinal Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455
The complementary nature of DNA allows this biomolecule to be an attractive scaffold in the design of self-assembled nanostructures. Specifically, DNA can aid in the patterning and precise assembly of protein molecules. To accomplish this, the protein must be functionalized with single-stranded DNA and subsequently hybridized to a complementary DNA backbone. Here, we employ a chemoenzymatic strategy to selectively modify proteins with an azide molecule. The azide-functionalized protein is then reacted with DNA containing an alkyne moiety using the click reaction. With our strategy, the protein of interest does not have to be pure and may contain multiple cysteines. These protein-DNA constructs may then be immobilized onto DNA backbones to create protein nanostructures. Applications of such protein-DNA conjugates include protein chips, biosensors, and nanofactories.
 

New Reactions and Methodology, Total Synthesis, Materials, Devices and Switches, Lipids, Nucleotides and Mimetics
8:00 PM-10:00 PM, Tuesday, March 27, 2007 Hyatt Regency Chicago -- Riverside Center, Poster

Division of Organic Chemistry

The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007