ORGN 756 |
| A sensor array containing six non-covalent gold nanoparticle-fluorescent polymer conjugates have been created to detect and identify protein targets. Cationic gold nanoparticles interact with carboxylate-functionalized poly(p-phenyleneethynylene)s through complementary electrostatic interactions, affording supramolecular complexes with varying stability. This complex formation leads to the fluorescence quenching of the polymer through an energy transfer to the gold nanoparticles. This sensor array was subject to the detection of seven proteins, including bovine serum albumin, cytochrome c, β-galactosidase, lipase, acid phosphatase, alkaline phosphatase, and subtilisin A. The presence of proteins disrupts the nanoparticle-polymer interaction, producing distinct fluorescence response patterns. These patterns are highly repeatable and are characteristic for individual proteins. Linear discriminant analysis was conducted to quantitatively identify the protein patterns, and an accuracy of 96% was obtained on randomly chosen protein samples based on a training matrix (6 nanoparticles x 7 proteins x 6 replicates). This work demonstrates the construction of novel nanomaterial-based protein detector arrays with potential applications in medical diagnostics. |
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Materials, Devices, and Switches
8:00 AM-12:00 PM, Thursday, March 29, 2007 McCormick Place Lakeside -- Room E450 A/B, Level 4, Oral
Division of Organic Chemistry |