ORGN 246 |
| This presentation will describe an approach to preparing peptide arrays with applications for screening small enzymatic biomolecules. Peptide arrays have a broad relevance in the biological sciences and offer opportunities in identifying protein binding interactions, enzyme activities and ligands that mediate cell adhesion. Recent work with the SPOT array methodology addresses many of these applications, but can be limited by the difficulty in developing suitable labeling strategies needed in assays. Here, a strategy is described that employs self-assembled monolayers as solid supports for on-chip peptide synthesis. The surfaces then permit the use of MALDI-MS to report the subsequent biological activity of enzymes and protein binding events. The approach has been automated to permit a rapid preparation and application of the arrays.
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Combinatorial and Process Chemistry
8:00 AM-11:40 AM, Monday, March 26, 2007 McCormick Place East -- Room E350, Level 3, Oral
Division of Organic Chemistry |
