Application of nanocapsules prepared from chitooligosaccharides and low molecular weight alginate

ORGN 625

Ting Wang, echo2165@163.com, Zhangqi Feng, fengzhangqi1981@163.com, and Nongyue He, nyhe1958@163.com. State Key Laboratory of Bioelectronics, Southeast University, Si Pai Lou 2, 210096 Nanjing, China
This report studied the application of a novel kind of nanocapsule 136 nm in diameter, synthesized from low molecular weight alginate (LMWALG) and chitooligosaccharides (COSs) in the quaternary W/O inverse micro emulsion system. BSA was used as model medication. The controlled-release mechanism of nanocapsule was studied and cumulative release curve was obtained, also loading conditions were optimized. The encapsulation efficiency could approach to 88.4% and sustained release characteration of the capsules was good during 24 h. The biocompatibility of the nanocapsule was studied by cytotoxicity experiment and the acute systemic toxicological test using two groups (negative and test) of mouses, each group have five ones. The results demonstrated that the survival rate of the cells ascended to 95.39% from 80.30% during three days and the symptom of toxicity was not found in negative control group, only one mouse presented simple hypokinesia at 4 hours after injection, nevertheless, hypokinesia disappeared at 18 hours.