COLL 400 |
| DNA and protein-based diagnostic assays are an important tool for clinicians because they provide improved methods for identifying genetic diseases, pathogens, disease-associated biomarkers, as well as determining an appropriate therapeutic regimen for patients. To meet future diagnostic testing demands, simpler and more rapid assays with improved sensitivity will be required. Nanosphere has developed DNA-modified gold nanoparticle probe technology to provide higher sensitivity and specificity detection of nucleic acid targets hybridized onto a substrate, enabling direct identification of SNPs without the use of PCR. Furthermore, this assay has been adapted to ultra-sensitive protein detection by co-labeling gold nanoparticles with antibodies and DNA barcodes. Following magnetic bead capture and nanoparticle binding, the DNA barcodes are released and detected with DNA-modified gold nanoparticle probes as described, providing > 3 orders of magnitude improvement in detection sensitivity over current methods. The development of assays and instrumentation, as well as applications of this technology, will be described. |
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Advances in Nanomedicine
8:30 AM-12:00 PM, Tuesday, 12 September 2006 Sir Francis Drake -- Monterey/Cypress Rooms, Oral
Division of Colloid & Surface Chemistry |