Applying imprint lithography (PRINT) for the generation of shape-specific, targeted nanocarriers in medicine

COLL 43

Julie A. DuPont, jadupont@email.unc.edu1, Larken E. Euliss, euliss@email.unc.edu2, Robert Z. Orlowski, R_Orlowski@med.unc.edu3, Jonathan Serody3, Klaus M. Hahn, khahn@med.unc.edu4, Christopher M Welch4, George W Small3, Ivana Ferrer3, and Joseph M. DeSimone, desimone@unc.edu5. (1) Department of Chemistry, University of North Carolina Chapel Hill, Chapel Hill, NC 27599, (2) Department of Chemistry, University of North Carolina at Chapel Hill, B-5 Venable Hall, Chapel Hill, NC 27599, (3) Department of Medicine, Division of Hematology/Oncology, University of North Carolina at Chapel Hill, CB 7295, Mason Farm Road, 22-003 Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599-7295, (4) Pharmacology, University of North Carolina, 1106 Mary Ellen Jones Building, CB# 7365, Chapel Hill, NC 27599, (5) Departments of Chemistry and Pharmacology, University of North Carolina at Chapel Hill, Department of Chemical Engineering, North Carolina State University, CB #3290 257 Caudill Laboratories, Chapel Hill, NC 27599
The emerging technology offered by Particle Replication In Non-wetting Templates (PRINT) provides an opportunity to take nanomedicine to the next level by incorporating all of the components of an ideal nanoparticle delivery vehicle. Specifically, being biocompatible, shape and size specific, monodisperse, composed of virtually any material, amenable to functionalization, and gentle enough for fragile biological cargo. We have successfully demonstrated the attachment of pendant chemical moieties onto the surface of the particles thorough the generic amine platform, carbonyldiimidazole (CDI). Specifically, the CDI conjugation permits the binding of the avidin-biotin conjugate to the surface of the PRINT particles which provides a tether through which a spectrum of further targeting proteins or antibodies can bind. Additionally, through a generic encapsulation technique, we have demonstrated that a chemotherapeutic can be encapsulated into the particle, creating a targeted nano-carrier which can deliver the chemotherapeutic in a controlled fashion.
 

Advances in Nanomedicine
8:30 AM-12:20 PM, Sunday, 10 September 2006 Sir Francis Drake -- Monterey/Cypress Rooms, Oral

Division of Colloid & Surface Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006