ORGN 593 |
| Phalloidin is a toxic, bicyclic heptapeptide found in the Death Cap mushroom (Amanita phalloides). When ingested, phalloidin binds to F-actin present in the liver and kidneys, decreasing the activity of these organs and causing renal failure and eventual death. Fluorescent phalloidin derivatives can be used to label cellular F-actin in vitro, enabling visualization of the cytoskeleton by fluorescence microscopy. This is convenient for the analysis of cell-based assays where changes to the actin cytoskeleton are being monitored. Here we describe a simple, high-yielding synthesis for a fluorescent phalloidin analog, featuring the novel use of a thioether-forming reaction.
|
|
New Reactions and Methodology, Heterocycles and Aromatics, Bioorganic Chemistry
8:00 PM-10:00 PM, Tuesday, 12 September 2006 Moscone Center -- Hall D, Poster
Sci-Mix
Division of Organic Chemistry |
