Synthetic ion channels as antibiotics

COLL 49

George W. Gokel, ggokel@wustl.edu, Department of Molecular Biology & Pharmacology, Washington University School of Medicine, 660 S. Euclid Ave., Campus Box 8103, St. Louis, MO 63110
Macrocyclic polyether compounds are excellent cation binders and their complexation behavior has been extensively studied during three decades. We have incorporated macrocycles into structures that we have developed for use as model cation-conducting transmembrane channels. These compounds transport H+, Na+, K+ through phospholipid membranes. Sodium transport has been assessed by dynamic 23Na-NMR methods and proton flux has been measured by fluorescence techniques. Sodium and potassium fluxes have been determined in certain cases by bilayer clamp methods in phospholipid bilayers. Hydraphile channels are currently thought to form water- and sodium-filled pores through the bilayer. As such, they may affect cellular osmotic balance when present in the plasma membrane. Toxicity has been correlated with channel length and some organism selectivity has been observed. The structure, function, and toxicity profiles of various synthetic ion channels will be discussed.
 

Advances in Nanomedicine
8:30 AM-12:20 PM, Sunday, 10 September 2006 Sir Francis Drake -- Monterey/Cypress Rooms, Oral

Division of Colloid & Surface Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006