Synthetic approach towards a protocell

ORGN 676

Michael S. DeClue, mdeclue@lanl.gov, Pierre-Alain Monnard, Gavin Collis, James A. Bailey, and Jim Boncella. Chemistry, Los Alamos National Laboratory, Los Alamos, NM 87545
The goal of the project is to transform inanimate molecules into an organized system resembling living units through a bottom-up approach. In particular, the coupling of two man-made chemical systems, one mimicking metabolism and the other heredity, inside of a dividing chemical compartment is needed. Our strategy tests if growth and division of pre-assembled fatty acid based vesicle systems are possible when provided synthetic precursors. Precursor molecules are designed to undergo photochemically catalyzed conversion into protocellular building blocks via a metabolic mediated process using a ruthenium organo-metal complex as a cofactor. Specifically, we are attempting to reduce picolinium ester based lipid like precursors into fatty acids with [Ru(bpy)3]2+ sensitized electron transfer from a sequence dependent nucleic acid. Synthetic efforts towards our fatty acid based protocell will be described along with preliminary experimental results that include the first example of photocatalytic cleavage of a picolinium ester to generate a carboxylic acid.