ORGN 18 |
| We have developed robust tools to study natural product biosynthetic proteins both in vitro and within cells. These techniques provide novel access labeling, visualization, and manipulation of any protein containing a carrier protein domain, including all polyketide, non-ribosomal peptide, and fatty acid synthases. In an effort to present these reporter-labeled precursors within live cells, we adapted reporter-labeled coenzyme A precursors to be taken up by the primary metabolism and covalently attached to proteins within the cell. This route now provides access to strategically-insert modifications within natural and engineered proteins. |
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Young Investigators Symposium
8:25 AM-12:00 PM, Sunday, 10 September 2006 Moscone Center -- Room 135, Oral
Division of Organic Chemistry |