COLL 401 |
| A central conundrum in diagnostics is the development of detection tools that are capable of high-level multiplexing, high sensitivity, excellent quantitation, AND can be used both in the point-of-care and central laboratory (high-throughput) settings. We have developed a series of nanoscale optical detection tags based on surface enhanced Raman scattering (SERS). These glass-coated, molecule-loaded gold nanoparticles are excited in the near-infrared, allowing detection in whole blood, and as many as a dozen different types can be simultaneously quantified using a handheld reader. The tags can also be detected in live animals using portable instrumentation. This presentation will highlight the basic properties of these novel nanomaterials, and will then describe our recently-developed multiplexed, quantitative lateral flow immunoassay (LFI) for Flu A, Flu B, and RSV. In all practical respects (time, sample volume, cartridge appearance, etc.), the assay is identical to typical rapid-test LFIs. However, in this case, the single test line contains all three capture Abs, and the readout involves a single beam interrogating a single spot. Preliminary, unoptimized results show LoDs by eye in the 30-50 ng/ml range for single antigens, a result better than can be obtained with commercially available kits. Using a portable reader and our proprietary analysis software, improved LoDs can be obtained for all three analytes simultaneously. Finally, we will highlight several additional applications under development. |
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Advances in Nanomedicine
8:30 AM-12:00 PM, Tuesday, 12 September 2006 Sir Francis Drake -- Monterey/Cypress Rooms, Oral
Division of Colloid & Surface Chemistry |