Study on the interaction between heparin/HS and proteins in hedgehog signaling pathway using surface plasmon resonance

CARB 108

Fuming Zhang, zhangf2@rpi.edu1, Jason S. McLellan2, Daniel J. Leahy2, and Robert J Linhardt, linhar@rpi.edu1. (1) Departments of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies, 110 8th Street, Troy, NY 12180, (2) Department of Biophysics & Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205
Heparin and heparan sulfate (HS) have been shown to interact with a number of biologically important proteins thereby playing an essential role in the regulation of various physiological processes. The understanding of these interactions at the molecular level is important for the design of new highly specific therapeutic agents. In addition, an understanding of specificity of heparin and heparan sulfate will be necessary to understand normal physiologic and pathophysiologic processes. Surface plasmon resonance (SPR) spectroscopy has been successfully used for quantitative modeling of heparin-protein interactions. In the present study, we report interactions between heparin/HS and proteins (hedgehog, and Ihog) in hedgehog signaling pathway using surface plasmon resonance.
 

General Contributed Papers: Synthesis
9:00 AM-11:00 AM, Thursday, 14 September 2006 Hilton San Francisco -- Yosemite C, Oral

Division of Carbohydrate Chemistry

The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006