POLY 229 |
| Nanometer-sized (<100 nm) polymer hydrogels (nanogels) have attracted growing interest due to the recent development of nanotechnology. There has also been interest in applying nanogels to drug delivery systems, such as protein delivery and gene delivery. We can develop tailor-made functional nanogels to create novel nanobiomaterials (nanogel engineering) by the self-assembly of functional associating polymers as building blocks. Several methods for in vitro intracellular protein delivery systems such as microinjection, modification of cell-penetrating peptide, cationic liposome systems and amphiphilic peptide based carriers were reported. However, the efficiency of cellular uptake is not always high. Here we investigated whether cationic choresterol-bearing (CHPNH2) nanogels can serve as intracellular protein or quantum dot carrier in comparison to conventional carrier systems. The cellular-uptake efficiency of CHPNH2 nanogels was higher than that of cationic liposome and peptide based carrier. CHPNH2-QD complex was effectively internalized by many kinds of cells without being aggregate. |
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7th International Biorelated Polymers Symposium
8:30 AM-12:40 PM, Monday, 11 September 2006 San Francisco Marriott -- Salon 14/15, Oral
Division of Polymer Chemistry |