Behavior of hydrophobized hyaluronane in aqueous solutions

CARB 64

Miloslav Pekar, pekar@fch.vutbr.cz, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic, Martin Chytil, Faculty of Chemistry, Brno University of Technology and CPN. Ltd, Purkynova 118, 612 00 Brno and 561 02 Dolni Dobrouc 401, Czech Republic, and Filip Mravec, Faculty of Chemistry, Brno University of Technology and CPN, Ltd, Purkynova 118, 612 00 Brno and 561 02 Dolni Dobrouc 401, Czech Republic.
Hyaluronic acid is important biopolymer playing significant role in human tissues and body fluids. It helps in nutrient transportation and affects cell behavior through interactions with specific proteins in the extracellular matrix and binding sites on the cell surface. Consequently, it is a promising candidate for targeted drug delivery. To improve its transport capability for non-polar molecules it has been modified by grafting alkyl chains. We report on solution behavior of dodecyl-modified hyaluronanes with degree of substitution between 3-5 and relative molecular weight within the range 32-97 000. Solutions were studied by viscosimetry, rheology, tensiometry and pyrene fluorescence. Viscosimetry and fluorescence confirmed aggregation behavior within the range 0.1 - 0.3 g/l, weakly dependent on the molecular weight. Surface activity of the modified hyaluronane is much higher than that of native biopolymer. Critical concentration from tensiometry is lower pointing to more complex aggregation mechanism than is known for classical surfactants.
 

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The 232nd ACS National Meeting, San Francisco, CA, September 10-14, 2006