Novel processes for synthesis of RNA oligonucleotides

CARB 70

Gang Wang, gwang@alnylam.com, Klaus Charisse, William Zedalis, Lubomir Nechev, and Muthiah Manoharan. Alnylam Pharmaceuticals, 300 Third Street, Cambridge, MA 02142
TEA∙3HF has been widely used to remove the tert-butyldimethylsilyl (TBDMS) protecting group from the 2' hydroxyl group of synthetic RNA oligonucleotides. We have developed alternate deprotection strategies to carry out this step. In this presentation, yields and impurity profiles obtained from these new methods will be compared to TEA∙3HF deprotection conditions.