CARB 11 |
| Antifreeze glycoproteins (AFGPs) are glycopolymers found in many different deep sea Teleost fish. The physiological role of these glycoproteins is to prevent in vitro ice growth and protect the organism against cell injury and death. On a fundamental level, these compounds have the ability to selectively depress the freezing point of an unfrozen solution (thermal hysteresis) and also prevent cellular damage from recrystallization in samples that have been frozen. These properties are very desirable for medical, industrial and commercial uses. While AFGPs have been extensively investigated as cryoadjuvants, the successes has been limited because thermal hysteresis activity increases the amount of cellular damage at temperatures outside of the thermal hysteretic gap (i.e. -5 oC and colder). Our laboratory has prepared a series of C-linked AFGP analogues that demonstrate recrystallization-inhibition (RI) activity equal to that of native AFGP but lack TH activity, making them ideal for cryopreservation applications. This is the first example where antifreeze activity of a biological antifreeze has been optimized for RI and the TH activity decoupled from that of the native system. This talk will describe the preparation of these analogues, SAR studies to elucidate the key structural features essential to RI activity and preliminary cellular applications. |
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Carbohydrate Recognition Mechanisms and Applications
9:00 AM-12:15 PM, Monday, 27 March 2006 Georgia World Congress Center -- B409, Oral
Division of Carbohydrate Chemistry |