Monosacchride arrays for the detection of bacterial adhesion by use of Quartz Crystal microbalance technique

CARB 49

Mingchuan Huang, mhuang@chemistry.ohio-state.edu1, Yun Zhang, yuzhang@chemistry.ohio-state.edu1, Peng George Wang, pgwang@chemistry.ohio-state.edu1, and Xiangqun Zeng, zeng@oakland.edu2. (1) Department of chemistry, the Ohio State University, 100 W. 18th Avenue, columbus, OH 43210, (2) Department of chemistry, Oakland University, 260 Science and Engineering Building,Rochester, Detroit, MI 48309
A simple method for rapid detection of bacterial adhesion by quartz crystal microbalance using carbohydrate arrays with self-assembled monolayers (SAMs) of alkanethiolates on gold was developed. The SAMs with mixtures of alpha-D-mannopyranoside and tri(ethylene glycol) moieties provide surfaces for studies of the adhesion of E. coli H5Dá and the binding of a lectin, concanavalin A(ConA) to the immobilized mannose. SAMs also provides excellent resistance to nonspecific adhesion.
 

General Posters
6:00 PM-8:00 PM, Tuesday, 28 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster

Division of Carbohydrate Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006