CARB 73 |
| Selenium derivatization has potential to play more and more important roles in determining the 3-D structures of nucleic acids and their complexes with proteins through X-ray crystallography using the multiwavelength anomalous dispersion (MAD) technique, because the heavy metal co-crystallization and halogen derivatization of nucleic acids often give unsatisfied results. Based on our previous research of the selenium-modified nucleosides, especially the demonstration of the stability of the selenium functionality and its compatibility with the solid-phase synthesis, we will report for the first time the novel synthesis of thymidine phosphoramidite derivatized at the exo-4 position with selenium, and present the investigation of its incorporation into the designed oligonucleotides using solid phase synthesis. This work is supported by NIH (GM069703) and NSF (0521553). |
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Oligonucleotides and Therapeutic Applications
9:00 AM-11:50 AM, Wednesday, 29 March 2006 Georgia World Congress Center -- B409, Oral
Division of Carbohydrate Chemistry |