CARB 37 |
| Inhibition of the mannose trimming enzyme human Golgi alfa-mannosidase II (HGMII), which acts late in the N-glycan processing pathway, provides one route to blocking the oncogene-induced changes in cell surface oligosaccharide structures. HGMII selectively cleaves alfa 1-3 and alfa 1-6 mannosyl residues present in its natural substrate GlcNAcMan5GlcNAc2. It has been proposed that HGMII has an extended binding site recognizing a large part of the oligosaccharide. In order to probe the substrate requirements of HGMII, we have synthesized a range of part-structures of GlcNAcMan5GlcNAc2 and determined kinetic parameters for hydrolysis by HGMII. The resulting information has been employed for the design of inhibitors for this enzyme. |
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General Posters
6:00 PM-8:00 PM, Tuesday, 28 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Carbohydrate Chemistry |