DNA sensing using nanoparticle-based receptors

PMSE 108

Belma Erdogan, belmae@chem.umass.edu1, Sai Archana Krovi, skrovi@student.umass.edu2, Gang Han, ghan@chemistry.umass.edu1, Ik-Bum Kim, ikbum.kim@chemistry.gatech.edu3, Uwe H. F. Bunz, uwe.bunz@chemistry.gatech.edu3, and Vincent Rotello, rotello@chem.umass.edu1. (1) Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, MA 01003, (2) Department of Chemistry, University of Massachusetts, Amherst, 710 North Pleasant St, 701 LGRT, Amherst, MA 01003, (3) School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA 30332-0400
Nanoparticles are extremely effective fluorescence quenchers, and provide an excellent dynamic range compared to small molecule-dye quenchers. Release of the fluorophores, which is a anionic poly(p-phenylene ethynylene) in our case, by using DNA and establishing DNA-nanoparticle assemblies results in almost complete fluorescence recovery further enhancing the sensitivity of the resulting sensors. An important feature of this sensing model is that instead of DNA, different proteins can be used and specific and selective binding of these proteins to either the fluorophore or the gold nanoparticles will generate a signal in highly sensitive manner.
 

Polymers, Nanoparticles and Composite Materials in Nanoscience
1:30 PM-4:50 PM, Monday, 27 March 2006 OMNI at CNN Center -- Intl. Blrm D, Oral

Division of Polymeric Materials: Science and Engineering

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006