Two-photon cellular imaging with high performance two-photon absorbing fluorescent probes

ORGN 597

Katherine J. Schafer-Hales, kschafe@mail.ucf.edu1, Kevin D. Belfield, belfield@mail.ucf.edu2, Sheng Yao, syao@mail.ucf.edu3, Peter K. Frederiksen4, Kiminobu Sugaya1, and Emmanuel Vrotsos1. (1) Department of Chemistry and Biomolecular Science Center, University of Central Florida, P.O. Box 162366, Orlando, FL 32816-2366, (2) Department of Chemistry and CREOL, College of Optics and Photonics, University of Central Florida, 4000 Central Florida Blvd., P.O. Box 162366, Orlando, FL 32816-2366, (3) Department of Chemistry, University of Central Florida, 4000 Central Florida Boulevard, P.O. Box 162366, Orlando, FL 32816-2366, (4) Department of Chemistry and College of Optics and Photonics: CREOL and FPCE, University of Central Florida, P.O. Box 162366, 4000 Central Florida Boulevard, Orlando, FL 32816-2366
Two-photon fluorescence imaging was demonstrated on both fixed and live NT2 cells incubated with new highly efficient 2PA fluorescent contrast agents. These probes high two-photon absorptivity, high fluorescence quantum yields, and high photostability, along with low cytotoxicity. The high performance of these contrast agents allowed two-photon fluorescence imaging using low irradiance over relatively long time periods (~24 h). Two-photon excitation conditions were investigated as a function of excitation wavelength and irradiance power. Two-photon spectroscopic properties and two-photon fluorescent microscopy images will be presented. The stability and sensitivity of these dyes makes them ideally suitable for multiphoton fluorescence imaging.
 

Heterocycles, Aromatics, Metal-Mediated Reactions and Syntheses, Materials, Devices, and Switches
8:00 PM-10:00 PM, Wednesday, 29 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- Ex. Hall B4, Sci-Mix

Division of Organic Chemistry

The 231st ACS National Meeting, Atlanta, GA, March 26-30, 2006