ORGN 396 |
| For the first time exo-4-selenium modified thymidine triphosphate (SeTTP) was successfully synthesized and efficiently incorporated into oligonucleotides. To determine the efficiency of this incorporation, several DNA templates were designed to show incorporation of exo-4-SeTTP at different positions. Efforts are underway to screen various DNA polymerases to determine the efficiency and stability of the duplex. To further improve on efficiency, we would explore a variety of conditions such as incubation time, salt condition, pH and the utilization of Mn2+ ions as the latter relaxes the specificity of many DNA polymerases. The findings of this study and contribution to structural elucidation will be presented.
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New Reactions and Methodology, Bioorganic Chemistry, Molecular Recognition and Self Assembly
8:00 PM-10:00 PM, Tuesday, 28 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Organic Chemistry |
