ORGN 423 |
| Synthetic riboswitches capable of activating protein translation in the presence of a particular small molecule have a variety of potential applications in synthetic chemistry and biotechnology. We recently reported a synthetic riboswitch that activates protein translation in response to the small molecule theophylline. While this switch was useful in both genetic screens and selections for the presence of theophylline, the dynamic range of the switch was limited. To address this limitation, we developed a high-throughput, microtiterplate-based assay that successfully identifies synthetic riboswitches that respond to a desired small molecule with improved dynamic ranges. These new switches display essentially no background translation in the absence of the small molecule ligand, and show large increases in translation in the presence of the ligand. Our new method may be employed to isolate synthetic riboswitches that respond to a variety of small-molecules. This method and its applications in biocatalysis will be presented. |
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New Reactions and Methodology, Bioorganic Chemistry, Molecular Recognition and Self Assembly
8:00 PM-10:00 PM, Tuesday, 28 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Organic Chemistry |