I&EC 131 |
| The use of waste material as an enzyme source is attractive with respect to the development of combined extraction-catalysis systems using ionic solvents. These systems have the potential to be cost effective and environmentally friendly. We studied the activity and stability of horseradish peroxidase (HRP) when extracted from waste horseradish using different ionic liquids including water immiscible [oMIM][PF6] and water miscible [4-MBP][BF4], [(eMIM)] [MeSO4] [eMIM][EtSO4] and [(oMIM)][Cl]. The water content of the solvent systems ranged from water-free to 25% water by volume in buffer (pH 7.4). Control solvents consisted of aqueous buffer, dioxane and tert-butanol. The extracted peroxidase enzyme exhibited high activity and stability in halogenated ionic solvents [(oMIM)][Cl] and [4-MBP][BF4]. The sulfate-based ionic liquids exhibited low activity suggesting enzyme deactivation via sulfide ion inhibition or some other mechanism. The loss of activity resulting from the action of the ionic liquids is partially reversible via aqueous buffer dilution.
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Ionic Liquids: Not Just Solvents Anymore OR Ionic Liquids: Parallel Futures (Sponsored by Green Chemistry and Engineering, Separation Science and Technology and Novel Chemistry with Industrial Applications Sub-Divisions)
8:00 PM-10:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Industrial and Engineering Chemistry |
