ORGN 131 |
| DNA sequencing is typically done by capillary array electrophoresis with the use of highly viscous polymer solutions ("gels") to separate DNA molecules according to size with single-base resolution. We demonstrate DNA sequencing by a different approach not requiring a gel, in which DNA molecules are tagged with monodisperse, water-soluble polypeptoids that enable free-solution capillary electrophoretic separation. For this purpose, we synthesized a novel class of branched, comb-like poly-N-substituted glycine drag-tags and attached them to DNA primers via a thiol-maleimide linkage. A linear, 30mer polypeptoid backbone with hydrophilic N-methoxyethyl (NMEG) side chains interspersed with reactive amino groups was synthesized by SPPS, HPLC-purified, and then derivatized via peptide bond formation with shorter, monodisperse oligoNMEGs to form monodisperse, comb-like conjugates. These drag-tags provide excellent DNA sequencing by free-solution capillary electrophoresis, up to about 100 bases. This family of branched drag-tags provides high frictional drag, even on a relatively short backbone. |
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Asymmetric Reactions and Syntheses, Physical Organic Chemistry, Combinatorial Chemistry, Total Synthesis
8:00 PM-10:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Division of Organic Chemistry |