ORGN 105 |
| Plasmid PUC19 was linearized using HIND III restriction enzyme. This linearized plasmid were cohesively ligated with T4 DNA ligase to an oligomer containing a covalently linked terminal anthraquinone group and six regularly spaced GG steps, which serve as traps for the migrating radical cation. Dynamic light-scattering results and examination by transmission electron microscopy (TEM) shows that the ligated DNA sample is converted into condensates by the addition of Spermidine. The condensates were irradiated with UV light (to introduce radical cations) and assayed by high resolution PAGE and phosphoimagery. DNA damage is found to be more localized at the site of initial electron loss compared with the corresponding oligomer in solution. The inhibition in the migration of radical cations is due to the decreased probability for forming the charge-transfer effective states that are required for radical-cation hoping. |
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Asymmetric Reactions and Syntheses, Physical Organic Chemistry, Combinatorial Chemistry, Total Synthesis
8:00 PM-10:00 PM, Sunday, 26 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix
Division of Organic Chemistry |