ORGN 522 |
| Fluorescent labels play an important role in a variety of biological and biotechnological applications, including the study of fluorescent conjugate interactions with their respective binding proteins or receptors. The choice of the fluorescent conjugate, which is influenced by the particular fluorescent chromophore utilized in conjugate preparation, can be critical for performance and cannot usually be made a priori. Of these dyes, the fluoresceins are the most commonly used labels due to their high solubility in aqueous buffers and high fluorescence quantum yield at physiological pH. While numerous fluorescein derivatives have been developed for conjugation to amine containing ligands, reactions of the aminofluoresceins with carboxy containing ligands suffer from poor reactivity and low yields. The greater reactivity of 5-aminoacetamidofluorescein makes this label suitable for carboxyl group conjugation. However, since no published synthesis of the label exists, we sought to develop a procedure, and describe here a rapid, economical preparation of 5-aminoacetamidofluorescein. |
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Heterocycles, Aromatics, Metal-Mediated Reactions and Syntheses, Materials, Devices, and Switches
8:00 PM-10:00 PM, Wednesday, 29 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Division of Organic Chemistry |