Enhance the enzyme stability through the addition of ionic liquids containing kosmotropic anions

CHED 223

Sophia Campbell, Jonathan Solomon, Artez Sims, Rasheed Lawal, Olarongbe Olubajo, and Hua Zhao. Chemistry Program, Savannah State University, Savannah, GA 31404
Hydrophobic ionic liquids based on [PF6]-, [Tf2N]- were investigated in a number of studies as reaction media for biocatalysis. However, there are only a few studies involving the use of water-containing hydrophilic ionic liquids for enzymatic reactions. Our research indicated that the ion hydration is very important to the enzyme activity and stability. Those kosmotropic anions such as [CH3COO]- and [CF3COO]- can enhance the enzyme stability in aqueous solutions. The chaotropic anion [BF4]- destabilizes the enzyme.