CHED 78

Introduction of ¹³C NMR spectrocopy to determine the isoelectric points of amino acids in aqueous solution

Jun H. Shin, jshin@qcc.cuny.edu¹, Sabrina M. Song, minsil2@hotmail.com¹, and Gopal Subramaniam, gsubrama@qc1.qc.edu². (1) Department of Chemistry, Queensborough Community College, 222-05, 56th Avenue, S-445, Bayside, NY 11364, (2) Department of Chemistry and Biochemistry, Queens College and the Graduate Center, CUNY, 65-30 Kissena Boulevard, Flushing, NY 11367

¹³C NMR spectroscopy was introduced to determine the isoelectric points (pI) of various types of amino acids (with neutral, basic or acidic side chain). The determination was carried out with Anasazi EFT 60 MHz FT-NMR Spectrometer, which was effective for the measurement because deuterated solvents were not required for the process. All the amino acid samples of various pHs were prepared with 1.5, 3 and 6 M HCl and NaOH mother solutions of the same amino acid concentration in deionized wate. The isoelectric points of the amino acids determined by ¹³C NMR spectroscopic method have been compared with the corresponding literature values.

Undergraduate Research Poster Session: Organic Chemistry
2:00 PM-4:00 PM, Monday, 29 August 2005 Washington DC Convention Center -- Hall A, Poster

Division of Chemical Education

The 230th ACS National Meeting, in Washington, DC, Aug 28-Sept 1, 2005