ORGN 733 |
| The rotation of a DNA nucleotide out of its original position in the duplex into a solvent exposed or enzyme-bound position (base flipping) has emerged as a common motif in many DNA repair reactions. The X-ray structure of E. coli CPD (cyclobutane pyrimidine dimer) photolyase shows that base flipping of the dimer into the active site is essential for the repair reaction. Despite base flipping being an important part of DNA repair experimental methods for studying this process are often difficult and require significant synthetic effort. A novel non-covalent assay for base flipping, in which the selective binding of a zinc-cyclen complex for pyrimidines is exploited, is presented. In order to detect binding solvatochromic dyes are linked to the zinc-cyclen complex. The fluorescence emission of the dansyl-cyclen complex responds to the change in environment from bulk water to the highly charged DNA environment. The synthesis and evaluation of this assay is presented.
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Lipids, Nucleotides, Biosynthesis, and Mimetics
8:00 AM-12:00 PM, Thursday, 1 September 2005 Washington DC Convention Center -- 202B, Oral
Division of Organic Chemistry |
