Towards homogeneous catalysis in organic solvent: PEG-modified subtilisin Carlsberg as a case study

ORGN 853

Yamaris Pacheco Moctezuma, yamarisp@hotmail.com1, Gabriel Barletta, gl_barletta@cuhac.upr.clu.edu2, and Kai Griebenow, griebeno@adam.uprr.pr1. (1) Department of Chemistry, University of Puerto Rico at Río Piedras, San Juan, PR 00926, (2) Chemistry, University of Puerto Rico, CUH Station, Humacao, PR 00791
The use of enzymes in organic solvents has become an important field during the last decade due to its advantages in the synthesis of chiral compounds of biological relevance. The modification of subtilisin Carlsberg with polyethylene glycol 20 kDa has been useful to solubilize the enzyme (up to 10 mg/mL) in some organic solvents like: acetonitrile, tetrahidrofurane, dichloromethane, dioxane and chloroform. This preparation is between 5,000 to 20,0000 folds more active than the enzyme's lyophilized powder. This is the highest increased of initial rate reported in literature so far using this type of system. Secondary and tertiary structural data and storage stability of the modified enzyme will be presented.
 

Asymmetric Reactions, Molecular Recognition, Self Assembly, Bioorganic Chemistry, Process R&D
8:00 PM-10:00 PM, Wednesday, 16 March 2005 Convention Center -- Hall D, Poster

Sci-Mix
8:00 PM-10:00 PM, Monday, 14 March 2005 Convention Center -- Sails Pavilion, Sci-Mix

Division of Organic Chemistry

The 229th ACS National Meeting, in San Diego, CA, March 13-17, 2005