ORGN 636 |
| RNA interference is driven through the recognition of small interfering RNAs (siRNA) by the RNA induced silencing complex (RISC). We are attempting to control RNA interference using light to allow us to control the spacing, timing and degree of gene expression in living cells. One strategy we are pursuing is to interrupt the interface between siRNA and the RISC using photo-labile groups attached to the siRNA duplex and in so doing, interrupt the recognition of the siRNA by the RISC. We have used the di-methoxy nitro phenyl ethyl group (DMNPE) to modify siRNAs and have demonstrated that these modified siRNAs have limited ability to effect RNA interference until irradiated. We will describe in detail our exploration of the effects of position and degree of modification on the degree to which RNA interference is modulated through the temporary blocking of siRNA/RISC interactions.
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Molecular Recognition and Self-Assembly
8:00 AM-12:00 PM, Wednesday, 16 March 2005 Convention Center -- Room 11A, Oral
Division of Organic Chemistry |