ORGN 311 |
| Dolly Batra1, Leilei Yin1, Ulrich Welp1, Philip D. Laible2, and Millicent A. Firestone3. (1) Materials Science Division and Center for Nanoscale Materials, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, (2) Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, (3) Center for Nanoscale Materials and Materials Science Division, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439 |
| The synthesis of biocompatible, nanostructured ‘soft’ materials that organize biomolecules into highly ordered, functional arrays is an important first step in the development of protein-based materials. A matrix comprising a 2-D hexagonal nanoporous network of crosslinked polyvinyl alcohol (PVA) has been fabricated and shown to exhibit potential for the integration and organization of soluble biomolecules (e.g., proteins). The matrix is prepared in a three step process. First, self-assembly of polystyrene (PS) beads on a Ni-chelate-functionalized substrate is employed to prepare a close-packed (2-D hexagonal) array. This framework serves as a template onto which a chemically crosslinked PVA thin film is cast. Finally, chemical etching of the PS spheres produces a highly ordered nanoporous polymeric network, which provides an affinity binding site for coupling of 6X-His-tagged protein. In this presentation, the application of this approach in the preparation of a 2-D array of green fluorescent protein (GFP) is described. The PS spheres and the PVA thin film are characterized by atomic force microscopy (AFM). The GFP array is analyzed via fluorescence spectroscopy and near-field scanning optical microscopy (NSOM). This work was performed under the auspices of the Office of Basic Energy Sciences, Division of Materials Sciences, United States Department of Energy, under Contract No. W-31-109-ENG-38. |
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Molecular Recognition and Materials
8:00 AM-12:00 PM, Tuesday, August 24, 2004 Pennsylvania Convention Center -- 201B, Oral
Division of Organic Chemistry |