ORGN 623 |
| Zhi Qiang Wang, Zhenjun Diwu, George G. Yi, and Jennie Francisco. Molecular Devices Corporation, 1311 Orleans Drive, Sunnyvale, CA 94089 |
| The application of regioisomerically pure fluorescent probes has been widely explored in biology such as labeling a biological molecule, locating proteins in living cells, detecting a specific functional fragment of proteins and measuring an intracellular ion concentration. In this presentation, a series of new reactive 5-carboxy-sulforhodamines have been synthesized with a relatively new tool of the microwave-accelerated synthesis. When 4-carboxy-2-sulfobenzaldehyde reacts with various N-substituted 3-aminophenols under microwave irradiation in DMF and TFA (10%), the condensation reaction generated a desired product with the cleaner products compared with the thermal reaction condition. The average yield is moderate in a range of 30-40%. These new reactive fluorescent dyes 5-carboxy-sulforhodamines emit different color such as green, orange and red (shown below). The conjugates of these reactive dyes with streptavidin have much higher brightness than relevant conjugates of existing dyes.
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New Reactions, Methodology, Heterocycles, Aromatics
8:00 PM-10:00 PM, Wednesday, August 25, 2004 Pennsylvania Convention Center -- Hall D, Poster
Division of Organic Chemistry |