Determination of the best procedure to isolate and characterize fatty acids from medicinal plants collected in Puerto Rico

CHED 808

Elba D. Reyes, Jaileen Rentas, Juan C. Flores, Yazmín Ortiz, Orly Santos, and Emanuel Vazquez. Department of Chemistry, University of Puerto Rico, Ave. Antonio R. Barceló, Cayey, PR 00736
For years it is known that some plants have therapeutic effects but, little is known about which component of these plants are responsible for the therapeutic effect. The lipid composition of medicinal plants in Puerto Rico is a very interesting research area, therefore we focus this research to found the best procedure to isolate and characterize the fatty acid compositions from a variety of medicinal plants in Puerto Rico. To obtain the crude extracts from each plant, specific quantity of leaves were triturated followed by sonication in dichloromethane (CH2Cl2) or extracted with methanol (MeOH), filtrated and rotoevaporated. Another procedure evaluated was Soxhlet extraction with different solvents such as hexane and methanol. The organic components of each crude extract were separated using chromatographic techniques. Derivatization reactions were performed to help in the GC-MS identification of the constituents. A quantitative comparative study of each procedures used for the isolation of the crude extract and the fatty acid methyl esters (FAME) composition from each plant will be presented.