CHED 810 |
| Rozalina Grubina, Zev J. Gartner, and David R. Liu. Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138 |
| Both in nature and in the laboratory, molecular evolution has been limited to nucleic acids, proteins, and other natural products. Recently, we have applied the principles of molecular evolution to the creation and discovery of complex synthetic small molecules using DNA-templated synthesis. To this end, we have translated a library of DNA sequences into corresponding libraries of small molecules. These DNA-small molecule conjugates are then selected in vitro for desired properties. We completed the synthesis of a 65 member pilot library of macrocyclic fumamarides from a library of 65 DNA templates. One of the macrocylic fumamarides was selected for binding to a target protein and its identity was confirmed by PCR amplification and sequencing of the encoding DNA template. The identities of other library members were analyzed by mass spectrometry. This work represents the first application of molecular evolution to the translation, selection, and amplification of DNA-encoded synthetic small molecules. |
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Undergraduate Research Poster Session: Organic Chemistry
2:00 PM-4:00 PM, Monday, March 29, 2004 Anaheim Convention Center -- Hall A, Poster
Division of Chemical Education |