ORGN 248 |
| Youngha Ryu, Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, TX 77840 and A. Ian Scott, Chemistry, Texas A & M University, College Station, TX 77843. |
| Ketosynthases are carbon-carbon forming enzymes in biosynthesis of fatty acids and polyketides. SELEX strategy is proposed for selecting ribozymes which could catalyze Claisen-type condensation which ketosynthases perform. A 220mer DNA pool which contains two random domains was constructed by mutual templating method from two synthetic single strand DNA templates and two primers. T7 RNA polymerase transcription, using a 5’-thiol modified guanosine analogue as an initiator nucleotide, provided an initial random RNA library, which in turn was chemically conjugated with an S-malonyl modifier. An S-acyl coenzyme A analogue bearing a biotin tag as a substrate was easily prepared by one-step conjugation. We will present the full synthetic procedures for preparing the substrates and chemical modifiers. Progress in an actual selection experiment using immobilized streptavidin will also be presented.
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Lipids, Biosynthesis, Enzyme Inhibitors, and Mimetics
8:00 AM-11:20 AM, Tuesday, March 30, 2004 Anaheim Convention Center -- 303D, Oral
Division of Organic Chemistry |