Determining binding constants for synthesized polyethylene glycol derivatized vancomycin derivatives and glycopeptides with use of affinity capillary electrophoresis

ORGN 489

Frank A. Gomez1, Lilia Hernandez2, and Marcellus M. Rudolph1. (1) Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032, (2) Department of Chemistry, California State University, Los Angeles, 5151 State Universtiy Dr, Los Angeles, CA 90022
Determining Binding Constants of Polyethylene glycol (PEG) derivatized vancomycin (Van) species and D-Ala-D-Ala terminus peptides using affinity capillary electrophoresis (ACE). In these experiments a series of Van-PEG (PEG-550, 750, 1100, 2000, 5000, and 8000 g/mol) species were synthesized. Utilizing ACE, a plug of Van-PEG and non-interacting standards are injected and electrophoresed. Analysis of the binding constant (Kb) demonstrate that derivatization of Van has little effect on the affinity of D-Ala-D-Ala peptide for Van. The findings help quantify the efficacy of PEG as a drug delivery system for glycopeptide antibiotics