We have developed a one-step synthesis of enantiomerically pure syn-1,3-diacetates from racemic diastereomeric mixtures of 1,3-diols that relies on: (i) an enzymatic resolution, (ii) a ruthenium-catalyzed epimerization and (iii) an intramolecular acyl migration. The in situ coupling of these three processes results in an efficient enantioselective synthesis of acyclic syn-1,3-diacetates and constitutes a novel concept for dynamic kinetic asymmetric transformation (DYKAT).