ORGN 503 |
| Hussam M. Bdour and John-Stephen A. Taylor. Department of Chemistry, Washington University, Campus Box 1134, One Brookings Drive, St. Louis, MO 63130-4899 |
| Cis-syn thymine dimers are the major photoproducts of DNA and are the principal cause of mutations induced by sunlight. To better understand the nature of base pairing with cis-syn thymine dimers we have synthesized 10-mer oligodeoxynucleotides (ODNs) containing a cis-syn dimer of TT labeled at N3 of both Ts. The ODNs were synthesized by two methods from 15N-labeled thymidine. In the first method an ODN containing an 15N-labeled TT site was irradiated and the cis-syn dimer isolated by HPLC. In the second method, an 15N-labeled cyclobutane thymine dimer phosphoramidite building block was first prepared and used to introduce the dimer into the ODN by solid phase automated automated synthesis. |
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Bioorganic, Molecular Recognition, Asymmetric Reactions and Syntheses
11:00 AM-1:00 PM, Wednesday, September 10, 2003 Javits Convention Center -- Hall 1B/1C, Poster
Division of Organic Chemistry |