ORGN 431 |
A model biosensor using the streptavidin-biotin system and self-amplifying conjugated polymers
| Juan Zheng and Timothy M. Swager. Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 |
Conjugated polymers such as poly(phenylene ethynylene)s (PPEs) provide a variety of methods for signal transduction in a sensing event, as changes in optical properties can occur as a function of pH, oxidation state and conformation. In an effort to harness their fluorescent properties in the detection of biological agents, we have synthesized a biosensor consisting of biotinylated (PPEs). The system makes use of the multivalent biotin-streptavidin interactions, which serve as a model for biological recognition. Quenching due to aggregation and fluorescent resonance energy transfer (FRET) are investigated as potential methods of signal transduction. The solution and solid phase sensing responses of the polymer are studied, in addition to analyte detection at the air-water interface.  |
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Physical Organic, Materials, Heterocycles, Aromatics, Metal-Mediated Reactions
8:00 PM-10:00 PM, Tuesday, September 9, 2003 Hilton New York -- Americas Hall 1, Poster
Division of Organic Chemistry
The 226th ACS National Meeting, New York, NY, September 7-11, 2003
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