Control of protein structure and function through surface recognition by tailored nanoparticle scaffolds

ORGN 575

Rui Hong1, Nicholas O. Fischer1, Ayush Verma1, Catherine McIntosh Goodman1, Todd Emrick2, and Vincent Rotello1. (1) Department of Chemistry, University of Massachusetts Amherst, 710 N. Pleasant St., Amherst, MA 01003, (2) Department of Polymer Science and Engineering, University of Massachusetts Amherst, 120 Governors Drive, Amherst, MA 01003
We have functionalized CdSe nanoparticles with thioalkylated polyethylene glycol (PEG) ligands (2, 3). These pegylated nanoparticles are highly water-soluble in a wide pH range from 3 to13 without aggregation. Using this family of particles, we have demonstrated several levels of control over chymotrypsin structure and function. Nanoparticles functionalized with 1 inhibit the activity of chymotrypsin and denature the protein. Dramatically reduced nonspecific adsorption of chymotrypsin onto nanoparticles was observed using sidechain 2 functionalized monolayer. Integration of PEG and recognition elements into the monolayer such as 3 provides tailored interactions between nanoparticles and proteins. Using these nanoparticles, inhibition of the enzymatic activity of chymotrypsin was observed with essentially no denaturation.

 

Bioorganic, Molecular Recognition, Asymmetric Reactions and Syntheses
11:00 AM-1:00 PM, Wednesday, September 10, 2003 Javits Convention Center -- Hall 1B/1C, Poster

Division of Organic Chemistry
The 226th ACS National Meeting, New York, NY, September 7-11, 2003