ORGN 317 |
| Laura A. MacManus-Spencer, Kristopher McNeill, Douglas E. Latch, and Kim M. Kroncke. Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455 |
| A sensitive and selective chemiluminescent probe has been developed and tested for the in situ detection of singlet oxygen (1O2), a transient species that displays cytotoxic effects in biological systems and is known to transform organic pollutants in the environment. Detection is based on the reaction of 1O2 with an electron-rich alkene to form the corresponding dioxetane, which undergoes chemiluminescent decomposition upon addition of a chemical trigger. The probe is designed as a trap-and-trigger detection method and is tethered to a polymer resin for in situ applications. Exposure of the alkene probe in the system of interest traps 1O2 to generate the stable dioxetane, and the chemiluminescent signal is triggered and quantified when such measurements are convenient. Due to the specificity of dioxetane formation by 1O2, as well as the low background and detection limits of chemiluminescence measurements, this probe is proposed to provide the most sensitive and selective in situ detection and quantitation of 1O2. |
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Materials, Devices, and Switches
8:00 AM-11:20 AM, Tuesday, September 9, 2003 Sheraton New York -- Versailles Ballroom, Oral
Division of Organic Chemistry |