ORGN 700 |
| Zhongfa Liu and Lawrence M. Sayre. Department of Chemistry, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106 |
| Modification of proteins by products of lipid oxidation, including 4-hydroxy-2-nonenal (HNE) and 4-oxo-2-nonenal (ONE), contributes to protein dysfunction in aging and degenative disease. Determining the nature of protein adduction by direct isolating and routine characterization using model systems under physiomimetic conditions is complicated by the complexity of the reactions and the reversiblity of many of the adducts. In the study, a chromatophoric and isotope-coded lysine surrogate, a 1:1 mixture of Na-(2,4-dinitrophenyl)lysine and its 3,5,6-trideuterio analog was employed to investigate reactions of HNE and ONE by HPLC electrospray ionization mass spectrometry. The method can differentiate cross-link from the non-cross-link modifications. From ONE, two previously identified cross-link and new oxidative adducts (a non-cross-link maleimide and a cross-link 3-aminosuccinimide) were identified. The latter were confirmed by a synthetic samples and detected in the reaction of ONE with tetrapeptide, Na-formyl-MLFK. |
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Proteins, Peptides, Amino Acids, and Nucleotides
8:30 AM-11:30 AM, Thursday, September 11, 2003 Sheraton New York -- Royal Ballroom B, Oral
Division of Organic Chemistry |